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LY6C + LY6G 抗体

Name: LY6C + LY6G 抗体
SKU: ABIN1177321
Availability: OutOfStock

This 大鼠单克隆 antibody specifically detects LY6C + LY6G in FACS, IHC (fro), WB, IP, IHC (f), IHC (zinc), CyTox 和 IHC (p). It exhibits reactivity toward 小鼠. It has been mentioned in 10+ publications

(10 references)

产品编号 ABIN1177321

发货至: 中国

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Quick Overview for LY6C + LY6G 抗体 (ABIN1177321)

抗原

LY6C + LY6G

适用

小鼠

宿主

大鼠

克隆类型

单克隆

标记

非结合性

应用范围

Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Cytotoxicity Test (CyTox), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB6-8C5

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

过滤

0.2 μm filtered

内毒素水平

Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

亚型

IgG2b kappa
anti-LY6C + LY6G antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE

anti-LY6C + LY6G antibody
适用: 小鼠 FACS, IHC, WB, Dep 宿主: 兔 Chimeric RB6-8C5 unconjugated Recombinant Antibody

anti-LY6C + LY6G antibody (FITC)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC

anti-LY6C + LY6G antibody
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 unconjugated

anti-LY6C + LY6G antibody (Cy5)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 Cy5

anti-LY6C + LY6G antibody (PE-Cy7)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7

anti-LY6C + LY6G antibody (Biotin)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 Biotin

anti-LY6C + LY6G antibody (APC-Cy7)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC-Cy7

anti-LY6C + LY6G antibody (PE-Cy5.5)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy5.5

anti-LY6C + LY6G antibody (SPRD)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 SPRD

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

储存液

Azide free

储存条件

4 °C

储存方法

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Nagendra, Schlueter: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, (2004) (PubMed).

Lagasse, Weissman: "Flow cytometric identification of murine neutrophils and monocytes." in: Journal of immunological methods, Vol. 197, Issue 1-2, pp. 139-50, (1996) (PubMed).

Tumpey, Chen, Oakes, Lausch: "Neutrophil-mediated suppression of virus replication after herpes simplex virus type 1 infection of the murine cornea." in: Journal of virology, Vol. 70, Issue 2, pp. 898-904, (1996) (PubMed).

Rakhmilevich: "Neutrophils are essential for resolution of primary and secondary infection with Listeria monocytogenes." in: Journal of leukocyte biology, Vol. 57, Issue 6, pp. 827-31, (1995) (PubMed).

Conlan, North: "Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 259-68, (1994) (PubMed).

Czuprynski, Brown, Maroushek, Wagner, Steinberg: "Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 4, pp. 1836-46, (1994) (PubMed).

Fleming, Fleming, Malek: "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2399-408, (1993) (PubMed).

Tepper, Coffman, Leder: "An eosinophil-dependent mechanism for the antitumor effect of interleukin-4." in: Science (New York, N.Y.), Vol. 257, Issue 5069, pp. 548-51, (1992) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).

Jutila, Kroese, Jutila, Stall, Fiering, Herzenberg, Berg, Butcher: "Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon-gamma." in: European journal of immunology, Vol. 18, Issue 11, pp. 1819-26, (1989) (PubMed).
抗原

LY6C + LY6G

别名

Ly-6G, Ly-6C

背景

The RB6-8C5 antibody reacts with a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 mAb is a component of the lineage cocktail used in studies of hematopoietic lineages. The mAb 1A8 (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C. Based on the comparison of the staining patterns of mAbs clones 1A8 and RB6-8C5 on total blood leukocytes, it is evident that mAb 1A8 stains the RB6-8C5-bright population, corresponding to Ly-6G-expressing granulocytes, whereas, the RB6-8C5-dim population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Please refer to the TDS Cat. No. 551459 and 553128 for more detail information.
Synonyms: Gr-1