IL-4 抗体
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北京 101111
Quick Overview for IL-4 抗体 (ABIN1177294)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-4. Neutralization activity may be measured with a proliferation assay using 1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to stimulate D36 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-4 for 60 minutes).
50% Neutralization (ND50) at 1.5 - 75 ng/mL
>95% Neutralization at 0.2 - 1.1 µg/mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Recombinant Mouse IL-4
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亚型
- IgG2b
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anti-Interleukin 4 (IL4) (AA 25-153) antibody
IL4 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150) antibodyIL4 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 61-111) antibodyIL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (Internal Region) antibodyIL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150), (AA 122-151), (C-Term) antibodyIL4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22885 unconjugated
anti-Interleukin 4 (IL4) antibodyIL4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 26-136) antibodyIL4 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) antibody (Biotin)IL4 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
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应用备注
- ELISA: The purified format of BVD4-1D11 antibody (Cat. No. 554387) can be useful as a capture antibody in a sandwich ELISA for measuring mouse IL-4. Purified BVD4-1D11 antibody can be paired with the biotinylated BVD6-24G2 antibody (Cat. No. 554390) as the detecting antibody, with recombinant mouse IL-4 (Cat. No. 550067) as the standard. For testing mouse IL-4 in complex biological fluids, such as serum or plasma, investigators may wish to consider using the BD OptEIA™ Mouse IL-4 Set (Cat. No. 555232).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, (1994) (PubMed).
: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).
: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL-4
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背景
- The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the BVD4-1D11 hybridoma was recombinant mouse IL-4. This is a neutralizing antibody.
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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