H2-T23 抗体 (AA 161-179)
Cited in 7+ publications. This anti-H2-T23 antibody is a 小鼠 单克隆 antibody detecting H2-T23 in FACS, IP, WB 和 BR. Suitable for 小鼠.
(7 references)
产品编号 ABIN1177269
发货至:
中国
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Quick Overview for H2-T23 抗体 (AA 161-179) (ABIN1177269)
抗原
See all H2-T23 抗体
H2-T23
(Histocompatibility 2, T Region Locus 23 (H2-T23))
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
This H2-T23 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Blocking Reagent (BR)
克隆位点
6A8-6F10-1A6
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抗原表位
- AA 161-179
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纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Qa-1[b] aa. 161-179 Peptide
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亚型
- IgG1 kappa
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anti-Histocompatibility 2, T Region Locus 23 (H2-T23) antibody
H2-T23 适用: 小鼠 FACS, IP, WB, Neut 宿主: 小鼠 Monoclonal 6A8-6F10-1A6 unconjugated
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应用备注
- Flow cytometry: Since this antigen is expressed at low density on the cell surface, it may be desirable to amplify staining by using a biotinylated second-step antibody (such as anti-mouse IgG1 mAb A85-1, Cat. No. 553441) followed by a bright third-step reagent such as PE Streptavidin (Cat. No. 554061).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." in: The Journal of experimental medicine, Vol. 190, Issue 12, pp. 1801-12, (2000) (PubMed).
: "T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5398-406, (1999) (PubMed).
: "The Qa-1b molecule binds to a large subpopulation of murine NK cells." in: European journal of immunology, Vol. 28, Issue 12, pp. 4356-61, (1999) (PubMed).
: "Cutting edge: expression of functional CD94/NKG2A inhibitory receptors on fetal NK1.1+Ly-49- cells: a possible mechanism of tolerance during NK cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 6976-80, (1999) (PubMed).
: "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." in: The Journal of experimental medicine, Vol. 188, Issue 10, pp. 1841-8, (1999) (PubMed).
: "Suppression of immune responses by CD8 cells. II. Qa-1 on activated B cells stimulates CD8 cell suppression of T helper 2 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 566-71, (1998) (PubMed).
: "Structural and functional characteristics of the class IB molecule, Qa-1." in: Immunological reviews, Vol. 147, pp. 67-89, (1996) (PubMed).
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: "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." in: The Journal of experimental medicine, Vol. 190, Issue 12, pp. 1801-12, (2000) (PubMed).
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- H2-T23 (Histocompatibility 2, T Region Locus 23 (H2-T23))
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别名
- Qa-1 b
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背景
- The 6A8.6F10.1A6 monoclonal antibody reacts with the Qa-1[b] alloantigen, which is a nonclassical MHC class I (Class Ib) molecule encoded by the T23 gene of the H-2 complex. Qa-1 associates with beta2-microglobulin and is expressed at low levels on most leukocytes and many other cell types. Its level of cell-surface expression is upregulated by IFNgamma or specific peptides. Qa-1 is an oligomorphic molecule which presents a limited pool of peptides to T lymphocytes bearing alphabeta and gammadeltaTCR and binds to a large subpopulation of NK cells. In fact, Qa-1[b] is the ligand for CD94/NKG2A, CD94/NKG2C, and CD94/NKG2E receptors, which are expressed on NK cells. Furthermore, it has been reported that Qa-1 expressed on activated B lymphocytes is involved in immunoregulation by inducing T-cell-mediated suppression of antibody responses. The 6A8.6F10.1A6 mAb can detect Qa-1[b] on activated splenocytes from C57BL/6 and BALB/c mice (both Qa-1[b]), but not from A/j mice (Qa-1[a]), and it can block target cell recognition by CTLs specific for Qa-1-presented antigen.
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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