(1 reference)-
- 抗原 See all CD22 抗体
- CD22 (CD22 Molecule (CD22))
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抗原表位
- pTyr822
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD22 antibody is conjugated to PE
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应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Mouse CD22 Peptide
- 克隆位点
- 12a-CD22
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD22 Primary Antibody
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anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 适用: 人 FACS, WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2H1C4 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat, StM 宿主: 小鼠 Monoclonal BLCAM-1795 unconjugated
anti-CD22 Molecule (CD22) (AA 621-725) antibodyCD22 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 1A3A11 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 FACS, WB, ELISA, IHC, IF, Coat 宿主: 小鼠 Monoclonal BLCAM-1796 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 适用: 人, Non-Human Primate FACS, WB, IP 宿主: 小鼠 Monoclonal MEM-01 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C3 unconjugated
anti-CD22 Molecule (CD22) (AA 20-687) antibodyCD22 适用: 人 FACS, WB, ELISA, IF, IP 宿主: 兔 Monoclonal DM13 unconjugated
anti-CD22 Molecule (CD22) antibody (PE)CD22 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-01 PE
anti-CD22 Molecule (CD22) antibody (FITC)CD22 适用: 人 FACS 宿主: 小鼠 Monoclonal IS7 FITC
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-022R unconjugated Recombinant Antibody
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- 样本量
- 20 μL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling." in: Annual review of immunology, Vol. 15, pp. 481-504, (1997) (PubMed).
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: "CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling." in: Annual review of immunology, Vol. 15, pp. 481-504, (1997) (PubMed).
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- 抗原
- CD22 (CD22 Molecule (CD22))
- 别名
- CD22 (CD22 产品)
- 背景
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CD22 is a member of the immunoglobulin (Ig) superfamily that contains several extracellular Ig domains and cytoplasmic Immunoreceptor Tyrosine-based Inhibitory Motifs (ITIMs). It is expressed in the cytoplasm of pro-B and pre-B cells and on the surface of IgD-positive mature B lymphocytes. Differential splicing of the human CD22 gene results in the expression of two isoforms, the full-length CD22b has 7 Ig domains, while CD22a has only 5. CD22b is the predominant isoform in human cells, at both the mRNA and protein levels. Ligands for the extracellular domains of CD22 are sialic acid-linked glycoproteins on the B cell surface. The intracellular ITIM motifs are substrates of src family tyrosine kinases. Signal transduction is initiated by the phosphorylation of tyrosines in the ITIMs of CD22 upon ligation of the B cell receptor (BCR). Tyrosine phosphorylation in ITIM3 of human CD22 or ITIM2 of mouse CD22 is required for recruitment of the protein tyrosine phosphatase PTP1C (SHP-1), which may terminate signal transduction by CD22 and by co-localized receptors, such as the BCR. Studies of mice deficient for CD22 suggest that this adhesion molecule generates both negative and positive regulatory signals that affect B cell development and responsiveness. The 12a/CD22 monoclonal antibody recognizes the phosphorylated tyrosine 822 (pY822) in ITIM3 of human CD22b. The equivalent site in human CD22a is pY645. The orthologous phosphorylation site in the predominant isoform of mouse CD22 is pY837, which is in ITIM2. We recommend the PE conjugate of mAb 12a/CD22, Cat. No. 560076, for staining both human and mouse leukocytes.
Synonyms: Human BL-CAM, Siglec-2, or Leu-14 (pY822), Mouse Lyb-8 (pY837)
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