Smad1 , Smad8 (AA 456-465), (pSer463), (pSer465) 抗体 (PE)
This 大鼠 单克隆 antibody specifically detects Smad1 , Smad8 in ICS. It exhibits reactivity toward 人 和 小鼠. It has been mentioned in 7+ publications
(7 references)
产品编号 ABIN1177177
发货至:
中国
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Quick Overview for Smad1 , Smad8 (AA 456-465), (pSer463), (pSer465) 抗体 (PE) (ABIN1177177)
抗原
Smad1 , Smad8
适用
人, 小鼠
宿主
大鼠
克隆类型
单克隆
标记
PE
应用范围
Intracellular Staining (ICS)
克隆位点
N6-1233
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抗原表位
- AA 456-465, pSer463, pSer465
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品牌
- BD Phosflow™
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纯化方法
- Purified from tissue culture supernatant or ascites by affinity chromatography
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免疫原
- Phosphorylated Human Smad1 aa 456-465 Peptide
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亚型
- IgG2a kappa
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样本量
- 5 μL
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实验流程
- In human PBMC, overnight serum starvation was found to be necessary for detection of a BMP-6-induced increase in Smad1/8 phosphorylation. Serum starvation for 1 hour following PBMC isolation was not sufficient to reduce basal levels of Smad1 (pS463/pS465)/Smad8(pS465/pS467). Some donor variation was observed in the reduction of basal phosphorylation by overnight serum starvation.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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: "BMP-6 inhibits human bone marrow B lymphopoiesis--upregulation of Id1 and Id3." in: Experimental hematology, Vol. 34, Issue 1, pp. 72-81, (2006) (PubMed).
: "BMP-6 inhibits growth of mature human B cells; induction of Smad phosphorylation and upregulation of Id1." in: BMC immunology, Vol. 6, pp. 9, (2005) (PubMed).
: "Smads and early developmental signaling by the TGFbeta superfamily." in: Genes & development, Vol. 12, Issue 16, pp. 2445-62, (1998) (PubMed).
: "Opposing BMP and EGF signalling pathways converge on the TGF-beta family mediator Smad1." in: Nature, Vol. 389, Issue 6651, pp. 618-22, (1997) (PubMed).
: "The TGF-beta family mediator Smad1 is phosphorylated directly and activated functionally by the BMP receptor kinase." in: Genes & development, Vol. 11, Issue 8, pp. 984-95, (1997) (PubMed).
: "Bone morphogenetic proteins: multifunctional regulators of vertebrate development." in: Genes & development, Vol. 10, Issue 13, pp. 1580-94, (1996) (PubMed).
: "MADR1, a MAD-related protein that functions in BMP2 signaling pathways." in: Cell, Vol. 85, Issue 4, pp. 489-500, (1996) (PubMed).
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- Smad1 , Smad8
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背景
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The N6-1233 monoclonal antibody specifically binds to the Smad1 protein phosphorylated at the Ser463/465 sites and the Smad8 protein phosphorylated at the Ser465/467 sites. Smad1 and Smad8 are ~60 kDa proteins and are members of the Smad superfamily. The Smad family members are divided into three subfamilies: receptor regulated Smads or R-Smads, including Smads1, 2, 3, 5, and 8, common partner Smad, or Co-Smad, including Smad4, and inhibitory Smads, or I-Smad, including Smads 6 and 7. Activation of Transforming Growth Factor-beta (TGF-beta) superfamily serine/threonine kinase receptors, such as TGF-beta and Bone Morphogenic Protein (BMP) receptors, leads to the phosphorylation of R-Smads at several sites. It has been shown that Ser463 and Ser465 of Smad1 are phosphorylated by BMP receptors. In B cells and pre-B cells, BMP-6 has been shown to induce Smad1/5/8 phosphorylation and inhibit cell proliferation. Phosphorylated R-Smads form complexes with Co-Smad and translocate into the nucleus to regulate transcription affecting a wide range of critical processes including cell-fate determination, proliferation, morphogenesis, differentiation and apoptosis. The inhibitory Smads inhibit this pathway through two potential mechanisms: either by preventing R-Smads from binding to their corresponding receptors and/or by competing with Smad4, the Co-Smad, from binding to R-Smads. This antibody may crossreact with Smad5 pS463/pS465 based on sequence homology.
Synonyms: Smad1,Smad8/Smad9, MADH1, MADH9
抗原
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