LCK 抗体 (pTyr505) (PE)
(2 references)Quick Overview for LCK 抗体 (pTyr505) (PE) (ABIN1177083)
抗原
See all LCK 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pTyr505
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品牌
- BD Phosflow™
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纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Phosphorylated Human Lck Peptide
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亚型
- IgG1
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anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 WB, ELISA, IP, RNAi 宿主: 小鼠 Monoclonal 3F7-F5 unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-240) antibodyLCK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 22-36) antibodyLCK 适用: 人 WB, IP, ICC, ICFC 宿主: 小鼠 Monoclonal LCK-01 unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 23-52) antibodyLCK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibodyLCK 适用: 人 ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 468-506), (C-Term) antibodyLCK 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 480-509) antibodyLCK 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 421-509) antibodyLCK 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 39-52) antibodyVerified LCK 适用: 人, 大鼠, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibodyLCK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells and cell lines (using BD Cytofix™ Fixation Buffer or BD™ Phosflow Fix Buffer I). Any of the three BD™ Phosflow permeabilization buffers may be used.
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样本量
- 20 μL
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "A supramolecular basis for CD45 tyrosine phosphatase regulation in sustained T cell activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 10138-43, (2000) (PubMed).
: "The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25429-32, (1997) (PubMed).
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: "A supramolecular basis for CD45 tyrosine phosphatase regulation in sustained T cell activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 10138-43, (2000) (PubMed).
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- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
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别名
- Lck
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背景
- Lck is a member of the Src family of cytoplasmic protein-tyrosine kinases (PTKs) that is normally expressed exclusively in lymphoid cells, primarily T lymphocytes and NK cells. Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell-surface glycoproteins of T lymphocytes, which recognize antigen presenting cells via their surface MHC class II and class I molecules, respectively. The catalytic activity of Lck is regulated by both kinases and phosphatases that control the phosphorylation states of two tyrosine residues that have opposing effects. Repression of Lck's catalytic activity occurs via phosphorylation at tyrosine 505 (Y505), located near the carboxy terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs, and its dephosphorylation is mediated by the protein tyrosine phosphatase, CD45. When Lck is phosphorylated at this site, it assumes a folded tertiary structure which is enzymatically inactive. When CD45 dephosphorylates it at Y505, Lck is able to autophosphorylate its Y394, which leads to conformational changes in the catalytic domain that induce kinase activity. However, it has been observed that the inhibitory effect of the phosphorylated Y505 can be overcome by direct engagement of Lck's SH3 domain and that both Y394 and Y505 are phosphorylated together in cells activated by hydrogen peroxide. Activated Lck phosphorylates the ITAMs (Immunoreceptor-based Tyrosine Activation Motifs) of the T cell receptor (TCR) and thus is critical for activation and development of T lymphocytes. The interactions of Lck, Csk, CD45, CD4 or CD8, and TCR are only a small part of a complex immunoregulatory cascade that involves additional substrates for Csk and CD45, other enzymes, adhesion molecules, adaptor proteins, and specialized membrane microdomains. The 4/LCK-Y505 monoclonal antibody recognizes the phosphorylated Y505 of the catalytic domain of Lck. The Alexa Fluor® 488- conjugated format has been evaluated by flow using a human model system. However, the unconjugated form of this antibody (Cat. No. 612390) has been shown to react with human, mouse, and rat in western blot. A phosphorylated peptide corresponding to residues around Tyrosine-505 from human Lck was used as the immunogen.
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
抗原
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