Btk and Itk (pTyr551) 抗体 (Alexa Fluor 647)
Cited in 1 publication. This anti-Btk and Itk antibody is a 小鼠 单克隆 antibody detecting Btk and Itk in ICS. Suitable for 人.
(1 reference)
产品编号 ABIN1177040
发货至:
中国
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Quick Overview for Btk and Itk (pTyr551) 抗体 (Alexa Fluor 647) (ABIN1177040)
抗原
Btk and Itk
适用
人
宿主
小鼠
克隆类型
单克隆
标记
Alexa Fluor 647
应用范围
Intracellular Staining (ICS)
克隆位点
24a-BTK
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抗原表位
- pTyr551
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品牌
- BD Phosflow™
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纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Phosphorylated Human Btk Peptide
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亚型
- IgG1 kappa
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样本量
- 20 μL
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
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: "Itk phosphorylation sites are required for functional activity in primary T cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37112-21, (2003) (PubMed).
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: "Itk phosphorylation sites are required for functional activity in primary T cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37112-21, (2003) (PubMed).
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- Btk and Itk
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背景
- Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. The activity of Btk is regulated by Src mediated phosphorylation of the kinase domain at tyrosine 551 (Y551). This event induces Btk kinase activity and subsequent autophosphorylation at Y223. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The Tec family kinase Itk plays a critical role in signal transduction downstream of the T cell antigen receptor and has been implicated in the activation of phospholipase C-gamma1 (PLCgamma1), a key regulator of calcium mobilization and extracellular signal-regulated kinase (ERK) activation. Itk is regulated by an activating transphosphorylation event in which Y511 in the kinase domain is phosphorylated by Lck. The 24a/BTK (Y551) monoclonal antibody recognizes the Y551-phosphorylated form of human Btk and the Y511 phosphorylated form of human Itk.
抗原
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