IL-4 抗体
(5 references)Quick Overview for IL-4 抗体 (ABIN1177017)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-4. Neutralization activity may be measured with a proliferation assay using 1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to stimulate CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-4 for 60 minutes).
50% Neutralization (ND50) at 1 - 50 ng/mL
>95% Neutralization at 50 - 300 ng/mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Partially Purified Mouse IL-4
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亚型
- IgG1
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anti-Interleukin 4 (IL4) (AA 25-153) antibody
IL4 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150) antibodyIL4 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 61-111) antibodyIL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (Internal Region) antibodyIL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150), (AA 122-151), (C-Term) antibodyIL4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22885 unconjugated
anti-Interleukin 4 (IL4) antibodyIL4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 26-136) antibodyIL4 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) antibody (PE)IL4 适用: 人, 猕猴 FACS 宿主: 大鼠 Monoclonal MP4-25D2 PE
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1357-67, (1995) (PubMed).
: "Enhanced IL-4-mediated D10.G4.1 proliferation with suboptimal concentrations of anti-IL-4 receptor monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 394-8, (1993) (PubMed).
: "Administration of anti-IL-4 monoclonal antibody 11B11 increases the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 3978-85, (1992) (PubMed).
: "IL-4 directs the development of Th2-like helper effectors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3796-806, (1991) (PubMed).
: "Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1." in: Nature, Vol. 315, Issue 6017, pp. 333-6, (1985) (PubMed).
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: "Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1357-67, (1995) (PubMed).
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL-4
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背景
- The 11B11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the 11B11 hybridoma was partially purified mouse IL-4 from PMA-stimulated EL-4 supernatant. The purified or unconjugated form of this antibody has been reported to be neutralizing.
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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