IL-5 抗体
(9 references)Quick Overview for IL-5 抗体 (ABIN1176999)
抗原
See all IL-5 (IL5) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- BD Pharmingen™
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产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-5. Neutralization activity may be measured with a proliferation assay using 1.5 ng/mL recombinant human IL-5 (Cat. No. 554606) to stimulate TF-1 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant human IL-5 for 60 minutes).
50% Neutralization (ND50) at 5 - 50 ng/mL
>95% Neutralization at 50 - 100 ng/mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Mouse Semi-Purified T-Cell
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亚型
- IgG1 kappa
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anti-Interleukin 5 (IL5) (AA 20-134) antibody
IL5 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 21-70) antibodyIL5 适用: 人, 小鼠, 大鼠, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 56-132) antibodyIL5 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 人 FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) (AA 20-134) antibodyIL5 适用: 人 WB 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 山羊 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 人 ELISA, D, ELISA (Capture) 宿主: 大鼠 Monoclonal JES1-39D10 unconjugated
anti-Interleukin 5 (IL5) antibodyIL5 适用: 小鼠 ELISA 宿主: 大鼠 Monoclonal TRFK5 unconjugated
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应用备注
- ELISA: The purified format of the TRFK5 antibody (Cat. No.554393) has been reported to be useful as a capture antibody for sandwich ELISA for measuring mouse or human IL-5. For a mouse IL-5 ELISA, purified TRFK5 antibody can be paired with the biotinylated TRFK4 antibody (Cat. No. 554397) as the detection antibody, with recombinant mouse IL-5 (Cat. No. 554581) as the standard. Alternatively, investigators may be interested in the BD OptEIA™ Mouse IL-5 ELISA Set (Cat. No. 555236). For a human IL-5 ELISA, purified TRFK5 antibody can be paired with the biotinylated JES1-5A10 antibody (Cat. No. 554491) as the detecting antibody, with recombinant human IL-5 (Cat. No. 554606) as the standard. Alternatively, investigators may be interested in the BD OptEIA™ Human IL-5 ELISA Set (Cat. No 555202).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Induction of cytokines in mice with parainfluenza pneumonia." in: Journal of virology, Vol. 69, Issue 2, pp. 1288-91, (1995) (PubMed).
: "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." in: Journal of immunological methods, Vol. 175, Issue 1, pp. 47-58, (1994) (PubMed).
: "Administration of anti-IFN-gamma antibody to beta 2-microglobulin-deficient mice delays influenza virus clearance but does not switch the response to a T helper cell 2 phenotype." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 3, pp. 1246-53, (1994) (PubMed).
: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
: "IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1." in: International immunology, Vol. 3, Issue 6, pp. 571-7, (1991) (PubMed).
: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
: "IL-4 directs the development of Th2-like helper effectors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3796-806, (1991) (PubMed).
: "The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 5, pp. 1576-81, (1988) (PubMed).
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
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- IL-5 (IL5) (Interleukin 5 (IL5))
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别名
- IL-5
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背景
- The TRFK5 antibody reacts with mouse interleukin-5 (IL-5) and cross-reacts with human IL-5. The TRFK5 antibody has been reported to cross react with IL-5 from rhesus monkey. This is a neutralizing antibody.
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
抗原
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