TLR4 /MD2 Complex 抗体
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北京 101111
Quick Overview for TLR4 /MD2 Complex 抗体 (ABIN1176997)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Mouse Pro-B cell line Ba/F3 expressing transfected BALB/c mouse TLR4 and MD-2
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亚型
- IgG2a kappa
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anti-TLR4 /MD2 Complex antibody (PE)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal MTS510 PE
anti-TLR4 /MD2 Complex antibody适用: 小鼠 FACS, IP, IHC, IHC (fro) 宿主: 大鼠 Monoclonal MTS510 unconjugated
anti-TLR4 /MD2 Complex antibody (Cy7,PE)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal MTS510 Cy7,PE
anti-TLR4 /MD2 Complex antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal MTS510 APC
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).
: "Identification of mouse MD-2 residues important for forming the cell surface TLR4-MD-2 complex recognized by anti-TLR4-MD-2 antibodies, and for conferring LPS and taxol responsiveness on mouse TLR4 ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 413-20, (2002) (PubMed).
: "The sole gateway to endotoxin response: how LPS was identified as Tlr4, and its role in innate immunity." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 29, Issue 4 Pt 2, pp. 474-8, (2001) (PubMed).
: "Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3471-5, (2000) (PubMed).
: "Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3476-9, (2000) (PubMed).
: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, (1999) (PubMed).
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: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).
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- TLR4 /MD2 Complex
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别名
- CD284/MD-2 Complex
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背景
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The MTS510 antibody reacts with the molecular complex of Toll-Like Receptor 4 and MD-2 (TLR4-MD-2) which is expressed on LPS responsive macrophages. TLR4, a member of the Toll-Like Receptor Family, has been renamed CD284 and identified to be the transmembrane signal-transducing portion of the receptor for LPS. TLR4 associates on the cell surface with CD14 and MD-2, a 0.7 kDa molecule which is anchored to the membrane via its physical association with TLR4. The association of MD-2 with TLR4 is required for recognition of LPS and the anti-mitotic compound Taxol, which mimics the action of LPS on mouse cells. MTS510 mAb detects TLR4-MD-2 on the surface of thioglycollate-elicited macrophages from all mouse strains tested (ie, BALB/c, C57BL/6, C3H/HeJ, C3H/HeN, and DBA/1), including the C3H/HeJ strain which expresses an LPS-resistant mutant TLR4. Expression of TLR4-MD-2 is down-regulated on peritoneal macrophages after exposure to LPS, correlating with the occurrence of LPS tolerance. TLR4-MD-2 is not detected on splenocytes or thymocytes.
Synonyms: TLR4/MD-2 Complex
抗原
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