IL-9 抗体
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北京 101111
Quick Overview for IL-9 抗体 (ABIN1176989)
抗原
See all IL-9 (IL9) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-9. Neutralization activity may be measured with a proliferation assay using 500 pg/mL recombinant mouse IL-9 to stimulate MC/9 cells at 5x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-9 for 60 minutes).
50% Neutralization (ND50) at 5 - 25 ng/mL
>95% Neutralization at 50 - 250 ng/mL -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Mouse IL-9 Recombinant Protein
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亚型
- IgG2 kappa
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anti-Interleukin 9 (IL9) (AA 21-120) antibody
IL9 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 9 (IL9) (AA 19-144) antibodyIL9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 9 (IL9) antibodyIL9 适用: 人, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 9 (IL9) (AA 19-144) antibodyIL9 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Interleukin 9 (IL9) (AA 19-144) antibodyIL9 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 9 (IL9) (Internal Region) antibodyIL9 适用: 人, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 9 (IL9) antibody (Biotin)IL9 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 9 (IL9) antibody (Biotin)IL9 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Interleukin 9 (IL9) (AA 74-123) antibodyIL9 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 9 (IL9) (AA 1-144) antibodyIL9 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA: Biotinylated D9302C12 antibody (Cat. No. 554473) is useful as a detection antibody in sandwich ELISA for measuring mouse IL-9 protein levels. Biotinylated D9302C12 antibody can be paired with the purified D8402E8 antibody (Cat. No. 551218) as the capture antibody, with recombinant mouse IL-9 as the standard.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Interleukin-9 and its receptor: involvement in mast cell differentiation and T cell oncogenesis." in: Journal of leukocyte biology, Vol. 57, Issue 3, pp. 353-60, (1995) (PubMed).
: "Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells." in: Nature, Vol. 291, Issue 5813, pp. 332-4, (1981) (PubMed).
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: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
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- IL-9 (IL9) (Interleukin 9 (IL9))
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别名
- IL-9
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背景
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The D9302C12 monoclonal antibody specifically binds to the multifunctional mouse cytokine, Interleukin-9 (IL-9). IL-9 is a 126 amino acid-long glycoprotein that is produced by various subsets of activated CD4+ T cells. IL-9 acts on target cells by binding to and signaling through the heterodimeric IL-9 receptor (IL-9R) complex that is comprised of transmembrane IL-9 receptor alpha (IL-9Ralpha) and common gamma chain (gammac) subunits. IL-9 can promote the survival, growth, proliferation and/or differentiation of various cell types including thymocytes, T cells, B cells, mast cells, and hematopoietic progenitor cells. IL-9 can augment IL-4-induced IgE and IgG1 production from lipopolysaccharide-primed mouse B cells and induce granzyme and high-affinity IgE receptor gene expression by mouse T helper cell clones and mast cell lines. IL-9 plays an important role in vivo in helminth elimination. The D9302C12 antibody neutralizes mouse IL-9 bioactivity.
Synonyms: IL-9, Interleukin-9, MEA, P40, T-cell growth factor P40, TCGF III -
途径
- JAK/STAT Signaling
抗原
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