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LCK 抗体 (N-Term) (Alexa Fluor 647)

Name: LCK 抗体 (N-Term) (Alexa Fluor 647)
SKU: ABIN1176916
Availability: OutOfStock

This anti-LCK antibody is a 小鼠单克隆 antibody detecting LCK in ICS. Suitable for 人和小鼠. This Primary Antibody has been cited in 1 publication.

(1 reference)

产品编号 ABIN1176916

发货至: 中国

Datasheet as PDF

Quick Overview for LCK 抗体 (N-Term) (Alexa Fluor 647) (ABIN1176916)

抗原

See all LCK 抗体

LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This LCK antibody is conjugated to Alexa Fluor 647

应用范围

Intracellular Staining (ICS)

克隆位点

MOL 171

Discover our top product LCK Primary Antibody

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N-Term

品牌

BD Phosflow™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human N-terminal Lck

亚型

IgG1 kappa
anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 WB, ELISA, IP, RNAi 宿主: 小鼠 Monoclonal 3F7-F5 unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-240) antibody
LCK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 22-36) antibody
LCK 适用: 人 WB, IP, ICC, ICFC 宿主: 小鼠 Monoclonal LCK-01 unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 23-52) antibody
LCK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 468-506), (C-Term) antibody
LCK 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 480-509) antibody
LCK 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 421-509) antibody
LCK 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 39-52) antibody
Verified LCK 适用: 人, 大鼠, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 1-539) antibody
LCK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

样本量

20 μL

限制

仅限研究用
状态

Liquid

缓冲液

Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Moroi, Koga, Nakamura, Ohtsu, Kimura, Nomoto: "Accumulation of p60lck in HTLV-I-transformed T cell lines detected by an anti-lck monoclonal antibody, MOL 171." in: Japanese journal of cancer research : Gann, Vol. 82, Issue 8, pp. 909-15, (1991) (PubMed).
抗原

LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

别名

Lck

背景

Lck is a member of the Src family of cytoplasmic protein-tyrosine kinases (PTKs) that is normally expressed exclusively in lymphoid cells, primarily T lymphocytes and NK cells. A low level of expression has been detected in B lymphocytes, but its function in B cells is unknown. Its expression in other leukocytes is not well defined. Members of the Src family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell-surface glycoproteins of T lymphocytes, which recognize antigen presenting cells via their surface MHC class II and class I molecules, respectively. The catalytic activity of Lck is regulated by both kinases and phosphatases that control the phosphorylation states of two tyrosine residues that have opposing effects. Repression of Lck's catalytic activity occurs via phosphorylation at tyrosine 505 (Y505), located near the carboxy terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs, and its dephosphorylation is mediated by the protein tyrosine phosphatase, CD45. When Lck is phosphorylated at this site, it assumes a folded tertiary structure which is enzymatically inactive. When CD45 dephosphorylates it at Y505, Lck is able to autophosphorylate its Y394, which leads to conformational changes in the catalytic domain that induce kinase activity. However, it has been observed that the inhibitory effect of the phosphorylated Y505 can be overcome by direct engagement of Lck's SH3 domain and that both Y394 and Y505 are phosphorylated together in cells activated by hydrogen peroxide. Activated Lck phosphorylates the ITAMs (Immunoreceptor-based Tyrosine Activation Motifs) of the T cell receptor (TCR) and thus is critical for activation and development of T lymphocytes. The interactions of Lck, Csk, CD45, CD4 or CD8, and TCR are only a small part of a complex immunoregulatory cascade that involves additional substrates for Csk and CD45, other enzymes, adhesion molecules, adaptor proteins, and specialized membrane microdomains. The MOL 171 monoclonal antibody recognizes the 56- and 60-kDa forms of human Lck protein, regardless of phosphorylation status. It cross reacts with mouse Lck.

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling