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B-Cell Linker 抗体 (N-Term) (Alexa Fluor 647)

Name: B-Cell Linker 抗体 (N-Term) (Alexa Fluor 647)
SKU: ABIN1176912
Availability: OutOfStock

This anti-B-Cell Linker antibody is a 小鼠单克隆 antibody detecting B-Cell Linker in ICS. Suitable for 人和小鼠. This Primary Antibody has been cited in 4+ publications.

(4 references)

产品编号 ABIN1176912

发货至: 中国

Quick Overview for B-Cell Linker 抗体 (N-Term) (Alexa Fluor 647) (ABIN1176912)

抗原

See all B-Cell Linker (BLNK) 抗体

B-Cell Linker (BLNK)

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This B-Cell Linker antibody is conjugated to Alexa Fluor 647

应用范围

Intracellular Staining (ICS)

克隆位点

2B11

Discover our top product BLNK Primary Antibody

抗原表位
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N-Term

品牌

BD Phosflow™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human N-terminal BLNK Recombinant Protein

亚型

IgG2a kappa
anti-B-Cell Linker (BLNK) antibody
BLNK 适用: 人, 小鼠 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5G9 unconjugated

anti-B-Cell Linker (BLNK) (AA 65-314) antibody
BLNK 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-B-Cell Linker (BLNK) (C-Term) antibody
Verified BLNK 适用: 人 WB, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated

anti-B-Cell Linker (BLNK) (AA 301-400) antibody
BLNK 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1D7 unconjugated

anti-B-Cell Linker (BLNK) (pTyr96) antibody
BLNK 适用: 人 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-B-Cell Linker (BLNK) (AA 41-337) antibody
BLNK 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-B-Cell Linker (BLNK) (N-Term) antibody
BLNK 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-B-Cell Linker (BLNK) (pTyr96) antibody
BLNK 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-B-Cell Linker (BLNK) (pTyr84) antibody
BLNK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-B-Cell Linker (BLNK) antibody
BLNK 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.

样本量

20 μL

限制

仅限研究用
状态

Liquid

缓冲液

Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Taguchi, Kiyokawa, Takenouch, Matsui, Tang, Nakajima, Suzuki, Shiozawa, Saito, Katagiri, Takahashi, Karasuyama, Matsuo, Okita, Fujimoto: "Deficiency of BLNK hampers PLC-gamma2 phosphorylation and Ca2+ influx induced by the pre-B-cell receptor in human pre-B cells." in: Immunology, Vol. 112, Issue 4, pp. 575-82, (2004) (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Li, Martin, Oliver, Kearney, Carter: "Antigen receptor proximal signaling in splenic B-2 cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3122-9, (2001) (PubMed).

Minegishi, Rohrer, Coustan-Smith, Lederman, Pappu, Campana, Chan, Conley: "An essential role for BLNK in human B cell development." in: Science (New York, N.Y.), Vol. 286, Issue 5446, pp. 1954-7, (1999) (PubMed).
抗原

B-Cell Linker (BLNK)

别名

BLNK

背景

B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The 2B11 monoclonal antibody recognizes BLNK, regardless of phosphorylation status. A fusion protein representing amino acids 4-205 of human BLNK was used as the immunogen. BLNK is expressed as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE that represent alternatively spliced forms of human BLNK.
Synonyms: SLP-65, BASH, BCA

途径

BCR Signaling