IL3RA 抗体
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北京 101111
Quick Overview for IL3RA 抗体 (ABIN1176902)
抗原
See all IL3RA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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过滤
- 0.2 μm filtered
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内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
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免疫原
- Human IL-3Ra-transfected cells
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亚型
- IgG2a kappa
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anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 200-305) antibody
IL3RA 适用: 人 FACS, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 8E11C5 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibody (PE)IL3RA 适用: 人 FACS 宿主: 小鼠 Monoclonal 6H6 PE
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-158) antibodyIL3RA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 201-300) antibodyIL3RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 167-331) antibodyIL3RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 1-100) antibodyIL3RA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 26-171) antibodyIL3RA 适用: 人 IHC, ELISA, Coat, ISt, StM 宿主: 兔 Monoclonal IL3RA-2947R unconjugated Recombinant Antibody
anti-Interleukin 3 Receptor, alpha (IL3RA) antibodyIL3RA 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Interleukin 3 Receptor, alpha (IL3RA) (AA 19-305) antibodyIL3RA 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM31 unconjugated
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应用备注
- This antibody has been found to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors and can neutralize IL-3 bioactivity. In a TF-1 cell proliferation assay, this antibody (at ~0.5 µg/ml) was found to inhibit by 50% the proliferation induced by 0.3 ng/ml (~1 U/ml) of human IL-3. In functional experiments, the 7G3 antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. In various formats, i.e., purified with azide (Cat. No. 554527), biotinylated (Cat. No. 554528), and phycoerythrin-labeled (Cat. No. 554529), the 7G3 antibody is useful for applications such as immunoprecipitation, Western blotting, and immunofluorescent labeling of cells for flow cytometric analysis.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, (1996) (PubMed).
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: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, (1996) (PubMed).
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- IL3RA (Interleukin 3 Receptor, alpha (IL3RA))
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别名
- CD123
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背景
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Reacts with human CD123, the 70 kD IL-3 receptor alpha chain (IL-3Ralpha), which associates with the 120-140 kD beta subunit. The beta chain is shared with the receptors for interleukins IL-5 and GM-CSF. IL-3Ralpha is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors. In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. This antibody has been reported to be useful for immunoprecipitation, western blot and immunofluorescent staining for flow cytometry. At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123. This antibody is routinely tested by LAL for endotoxin level and by immunofluorescent staining and flow cytometry.
Synonyms: IL-3 Receptor alpha chain -
途径
- JAK/STAT Signaling
抗原
-
