(1 reference)-
- 抗原 See all ENOS (NOS3) 抗体
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
-
抗原表位
- AA 1025-1203
-
适用
- 人, 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This ENOS antibody is conjugated to PE
-
应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human eNOS aa. 1025-1203
- 克隆位点
- 33-eNOS
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NOS3 Primary Antibody
-
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody
NOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1105-1202) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1-120) antibodyNOS3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (C-Term) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) antibodyNOS3 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 1156-1183) antibodyNOS3 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pThr495) antibodyNOS3 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (AA 756-888) antibodyNOS3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (Thr180), (Tyr182) antibodyNOS3 适用: 人, 大鼠, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
- 样本量
- 20 μL
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
-
-
: "Human cytomegalovirus inhibits Akt-mediated eNOS activation through upregulating PTEN (phosphatase and tensin homolog deleted on chromosome 10)." in: Cardiovascular research, Vol. 69, Issue 2, pp. 502-11, (2006) (PubMed).
-
: "Human cytomegalovirus inhibits Akt-mediated eNOS activation through upregulating PTEN (phosphatase and tensin homolog deleted on chromosome 10)." in: Cardiovascular research, Vol. 69, Issue 2, pp. 502-11, (2006) (PubMed).
-
- 抗原
- ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
- 别名
- eNOS (NOS3 产品)
- 背景
-
Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and both are regulated in a similar manner. The human forms exhibit 52% amino acid identity. However, they are distinct gene products of about 155 kDa (nNOS) and 140 kDa (eNOS). The eNOS gene was cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC). eNOS protein has a unique N-myristylation consensus sequence that may explain its membrane localization. The 33/eNOS monoclonal antibody recognizes eNOS, regardless of phosphorylation status. The specificity of this antibody conjugate for flow cytometric analysis was validated by confirming that RNA-mediated interference (RNAi) of the specific protein reduced the staining of the cells (see figure). Furthermore, the capacity of the RNAi to down-regulate the expression of the relevant protein was confirmed by western blot analysis.
Synonyms: NOS type III, NOS3, EC-NOS, NOS III - 途径
- ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
-
You are here:
