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IL7R 抗体

Name: IL7R 抗体
SKU: ABIN1176855
Availability: OutOfStock

Cited in 9+ publications. This anti-IL7R antibody is a 大鼠单克隆 antibody detecting IL7R in FACS, WB, IHC, IP 和 BR. Suitable for 小鼠.

FACS

1 image

(9 references)

产品编号 ABIN1176855

发货至: 中国

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Quick Overview for IL7R 抗体 (ABIN1176855)

抗原

See all IL7R 抗体

IL7R (Interleukin 7 Receptor (IL7R))

适用

小鼠

宿主

大鼠

克隆类型

单克隆

标记

This IL7R antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Blocking Reagent (BR)

克隆位点

SB-14

Discover our top product IL7R Primary Antibody

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

过滤

0.2 μm filtered

内毒素水平

Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

免疫原

BALB/c mouse pre-B cell line 1A9

亚型

IgG2a kappa
anti-Interleukin 7 Receptor (IL7R) (AA 173-260) antibody
IL7R 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 7 Receptor (IL7R) (AA 21-239) antibody
IL7R 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3F5D9 unconjugated

anti-Interleukin 7 Receptor (IL7R) (AA 21-239) antibody
IL7R 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 7 Receptor (IL7R) (N-Term) antibody
IL7R 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 7 Receptor (IL7R) (AA 21-236) antibody
IL7R 适用: 人 WB, ELISA, Coat 宿主: 小鼠 Monoclonal IL7R-2751 unconjugated

anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody
IL7R 适用: 人 FACS, WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 7 Receptor (IL7R) antibody
IL7R 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated

anti-Interleukin 7 Receptor (IL7R) antibody (APC)
IL7R 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal A7R34 APC

anti-Interleukin 7 Receptor (IL7R) (Ser552) antibody
IL7R 适用: 人, 小鼠, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 7 Receptor (IL7R) (AA 1-459) antibody
IL7R 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated

应用备注

Flow cytometry: Since this antigen is expressed at low density on lymphoid cell surfaces, it may be desirable to amplify staining by using a bright second-step reagent. If a three-step method is employed, the use of anti-Mouse CD16/CD32, Mouse Fc Block™ (Cat. No. 553141) is recommended to reduce non-specific staining. Special precaution should be taken to utilize a second-step antibody which does not crossreact with 2.4G2 mAb (rat IgG2b, kappa), such as biotinylated anti-rat IgG2a (RG7/1.30, Cat. No. 553894) followed by PE Streptavidin (Cat. No. 554061).

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

储存液

Azide free

储存条件

4 °C

储存方法

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Akashi, Kondo, Weissman: "Role of interleukin-7 in T-cell development from hematopoietic stem cells." in: Immunological reviews, Vol. 165, pp. 13-28, (1999) (PubMed).

Borghesi, Yamashita, Kincade: "Heparan sulfate proteoglycans mediate interleukin-7-dependent B lymphopoiesis." in: Blood, Vol. 93, Issue 1, pp. 140-8, (1999) (PubMed).

Fujihashi, Kawabata, Hiroi, Yamamoto, McGhee, Nishikawa, Kiyono: "Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3613-8, (1996) (PubMed).

Noguchi, Nakamura, Russell, Ziegler, Tsang, Cao, Leonard: "Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor." in: Science (New York, N.Y.), Vol. 262, Issue 5141, pp. 1877-80, (1994) (PubMed).

Peschon, Morrissey, Grabstein, Ramsdell, Maraskovsky, Gliniak, Park, Ziegler, Williams, Ware, Meyer, Davison: "Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1955-60, (1994) (PubMed).

Faust, Saffran, Toksoz, Williams, Witte: "Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells." in: The Journal of experimental medicine, Vol. 177, Issue 4, pp. 915-23, (1993) (PubMed).

Sudo, Nishikawa, Ohno, Akiyama, Tamakoshi, Yoshida, Nishikawa: "Expression and function of the interleukin 7 receptor in murine lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 9125-9, (1993) (PubMed).

Henderson, Narayanan, Collins, Dorshkind: "Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 1973-9, (1992) (PubMed).

Goodwin, Friend, Ziegler, Jerzy, Falk, Gimpel, Cosman, Dower, March, Namen: "Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily." in: Cell, Vol. 60, Issue 6, pp. 941-51, (1990) (PubMed).
抗原

IL7R (Interleukin 7 Receptor (IL7R))

别名

CD127

背景

The SB/14 monoclonal antibody reacts with CD127, the 65-75-kDa type-I transmembrane protein IL-7Ralpha. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Ralpha and CD132, the common gamma chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Interaction between IL-7 and its receptor is important for the proliferation of pre-B lymphocytes and can also trigger proliferation of CD4-CD8- immature thymocytes, as well as mature T cells in the periphery. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities. IL-7Ralpha is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density gammadelta TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring alphabeta TCR-bearing T cells. The SB/14 mAb neutralizes IL-7-induced proliferation of the IL-7-dependent immature B-lymphocyte line 2E8 (detected by BrdU incorporation). Blocking of IL-7-induced lymphocyte proliferation. 2E8 cells (mouse immature B lymphocyte cell line) were cultured for 48 hours in the presence of recombinant mouse IL-7 (blue histogram), with mAb SB/14 added 30 minutes prior to IL-7 (red histogram), or in the absence of IL-7 (green histogram). Proliferation was detected by incorporation of bromodeoxyuridine (BrdU) using the BrdU Flow Kit, Cat. No. 559619. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
Synonyms: IL-7 Receptor alpha chain

途径

JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size