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CD9 抗体

Cited in 10+ publications. This 大鼠 单克隆 anti-CD9 antibody (Clone KMC8) specifically detects CD9 in FACS, WB, IF, IP, IHC (fro) 和 BR. The antibody is reactive with 小鼠 samples.
产品编号 ABIN1176843
发货至: 中国
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中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD9 抗体 (ABIN1176843)

抗原

See all CD9 抗体
CD9

适用

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小鼠

宿主

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大鼠

克隆类型

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单克隆

标记

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This CD9 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

克隆位点

KMC8
  • 纯化方法

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    过滤

    0.2 μm filtered

    内毒素水平

    Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

    免疫原

    (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal cell line BMS2

    亚型

    IgG2a kappa
  • 限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.0 mg/mL

    缓冲液

    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

    储存液

    Azide free

    储存条件

    4 °C

    储存方法

    Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Nakorn, Miyamoto, Weissman: "Characterization of mouse clonogenic megakaryocyte progenitors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 205-10, (2003) (PubMed).

    Kaji, Takeshita, Miyake, Takai, Kudo: "Functional association of CD9 with the Fc gamma receptors in macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3256-65, (2001) (PubMed).

    Tachibana, Hemler: "Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance." in: The Journal of cell biology, Vol. 146, Issue 4, pp. 893-904, (1999) (PubMed).

    Tanio, Yamazaki, Kunisada, Miyake, Hayashi: "CD9 molecule expressed on stromal cells is involved in osteoclastogenesis." in: Experimental hematology, Vol. 27, Issue 5, pp. 853-9, (1999) (PubMed).

    Park, Yashiro, Tai, Toyo-oka, Hamaoka, Yagita, Okumura, Neben, Fujiwara: "Differential involvement of a Fas-CPP32-like protease pathway in apoptosis of TCR/CD9-costimulated, naive T cells and TCR-restimulated, activated T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 5790-6, (1998) (PubMed).

    Pulendran, Lingappa, Kennedy, Smith, Teepe, Rudensky, Maliszewski, Maraskovsky: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, (1997) (PubMed).

    Oritani, Wu, Medina, Hudson, Miyake, Gimble, Burstein, Kincade: "Antibody ligation of CD9 modifies production of myeloid cells in long-term cultures." in: Blood, Vol. 87, Issue 6, pp. 2252-61, (1996) (PubMed).

    Wright, Tomlinson: "The ins and outs of the transmembrane 4 superfamily." in: Immunology today, Vol. 15, Issue 12, pp. 588-94, (1995) (PubMed).

    Jennings, Crossno, Fox, White, Green: "Platelet p24/CD9, a member of the tetraspanin family of proteins." in: Annals of the New York Academy of Sciences, Vol. 714, pp. 175-84, (1994) (PubMed).

    Miyake, Medina, Hayashi, Ono, Hamaoka, Kincade: "Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 477-88, (1990) (PubMed).

  • 抗原

    CD9

    别名

    CD9

    背景

    The KMC8 antibody reacts with CD9, a 24-kDa member of the transmembrane 4 superfamily. In the mouse, CD9 is present on bone marrow myeloid cells, stromal cells, and megakaryocyte-committed progenitors, subsets of peripheral T and B lymphocytes, and neutrophils, platelets, dendritic cells, and bone marrow-derived macrophages. CD9 has been found to be associated with integrins and other cell-surface receptors, and it is suggested to play a role in signal transduction and possibly in regulating cellular adhesive properties. It has also been demonstrated to participate in T-cell costimulation and induction of apoptosis. The KMC8 antibody has been reported to block certain CD9 functions and activate macrophages

    途径

    Response to Water Deprivation, Cell-Cell Junction Organization
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