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BLMH 抗体 (AA 213-447) (Biotin)

BLMH 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal Biotin
产品编号 ABIN1175600
发货至: 中国
  • 抗原 See all BLMH 抗体
    BLMH (Bleomycin Hydrolase (BLMH))
    抗原表位
    • 15
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 213-447
    适用
    • 29
    • 28
    • 22
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 41
    • 11
    • 1
    克隆类型
    • 45
    • 8
    多克隆
    标记
    • 25
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BLMH antibody is conjugated to Biotin
    应用范围
    • 46
    • 13
    • 13
    • 12
    • 9
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    序列
    MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS- MEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAW
    特异性
    It has been selected for its ability to recognize BLMH in immunohistochemical staining and Western blotting.
    纯化方法
    Affinity Chromatography
    免疫原
    Recombinant BLMH expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against BLMH conjugated to biotin.
    亚型
    IgG
    Top Product
    Discover our top product BLMH Primary Antibody
  • 应用备注
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    说明

    Content: The quality control contains recombinant BLMH (Met213~Trp447) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freeze/thaw cycles
    储存条件
    4 °C
    储存方法
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    有效期
    12 months
  • 抗原
    BLMH (Bleomycin Hydrolase (BLMH))
    Abstract
    BLMH 产品
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