KRT16 抗体 (AA 117-419) (Biotin)
Quick Overview for KRT16 抗体 (AA 117-419) (Biotin) (ABIN1172995)
抗原
See all KRT16 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 117-419
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原理
- Biotin-Linked Polyclonal Antibody to Cytokeratin 16 (CK16)
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序列
- MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDD DDKAMADIGSEF-EKVT MQNLNDRLAS YLDKVRALEE ANADLEVKIR DWYQRQRPSE IKDYSPYFKT IEDLRNKIIA ATIENAQPIL QIDNARLAAD DFRTKYEHEL ALRQTVEADV NGLRRVLDEL TLARTDLEMQ IEGLKEELAY LRKNHEEEML ALRGQTGGDV NVEMDAAPGV DLSRILNEMR DQYEQMAEKN RRDAETWFLS KTEELNKEVA SNSELVQSSR SEVTELRRVL QGLEIELQSQ LSMKASLENS LEETKGRYCM QLSQIQGLIG SVEEQLAQLR CEMEQQSQEY QILLDVKTRL EQEIATYRR
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特异性
- The antibody is a rabbit polyclonal antibody raised against CK16. It has been selected for its ability to recognize CK16 in immunohistochemical staining and western blotting.
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
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Recombinant KRT16 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against KRT16 conjugated to biotin. -
亚型
- IgG
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应用备注
- Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freeze/thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- KRT16 (Keratin 16 (KRT16))
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别名
- Cytokeratin 16
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背景
- KRT16, NEPPK, Keratin 16, Focal Non-Epidermolytic Palmoplantar Keratoderma, Keratin, type I cytoskeletal 16
抗原
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