Complement C4c 抗体 (cleaved) (Biotin)
-
- 抗原 See all Complement C4c (C4c) products
- Complement C4c (C4c) (Complement Component C4c (C4c))
-
抗原表位
- cleaved
- 适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Complement C4c antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- 特异性
- It has been selected for its ability to recognize C4c in immunohistochemical staining and Western blotting.
- 纯化方法
- Affinity Chromatography
- 免疫原
-
Native Protein C4cC4b was cleaved by C3b inactivator (C3bINA) in the presence of C4b-binding protein and C4d and C4c isolated. The radioactive label and therefore the reactive thiol group were located to C4d. C4c was reduced and alkylated and the two alpha'-chain fragments of C4c were separated. The molecular weights, amino acid analyses and carbohydrate content of the three alpha'-chain fragments were determined. C4d has a mol.wt. of 44500 and a carbohydrate content of 6 % . The two alpha'-chain fragments of C4c have mol.wts. of 25000 and 12000 and carbohydrate contents of 10 and 22 % respectively.
The antibody is a rabbit polyclonal antibody raised against C4c conjugated to biotin. - 亚型
- IgG
-
anti-Complement Component C4c (C4c) antibody
C4c 适用: 人 WB, IHC, FACS, ELISA, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 10-12 unconjugated
anti-Complement Component C4c (C4c) (AA 19-333) antibodyC4c 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Complement Component C4c (C4c) (AA 19-333) antibodyC4c 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Complement Component C4c (C4c) (AA 680-756) antibodyC4c 适用: 人 WB, IHC, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze/thaw cycles
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- 有效期
- 12 months
-
- 抗原
- Complement C4c (C4c) (Complement Component C4c (C4c))
- 别名
- Complement Component 4c (C4c) (C4c 产品)
-
