Avidin 抗体 (Biotin)
Quick Overview for Avidin 抗体 (Biotin) (ABIN116961)
抗原
See all Avidin (AVD) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
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产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
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纯化方法
- A multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below
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免疫原
- Avidin [Hen Egg White]
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anti-Avidin (AVD) antibody (Cy3)
AVD 适用: Chicken egg IHC (fro), IHC (p), ICC 宿主: Chicken eggs Polyclonal Cy3
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, DB, IP 宿主: 兔 Polyclonal unconjugated
anti-Avidin (AVD) antibodyAVD 适用: Chicken egg WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 μg of Avidin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 32,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 10.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Do NOT add sodium azide! Dilute only prior to immediate use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.
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- Avidin (AVD)
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别名
- Avidin
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背景
- The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
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基因ID
- 396260
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NCBI登录号
- NP_990651
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UniProt
- P02701
抗原
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