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PDPK1 抗体 (AA 301-312)

Name: PDPK1 抗体 (AA 301-312)
SKU: ABIN116939
Availability: OutOfStock

The 兔多克隆 anti-PDPK1 antibody is suitable to detect PDPK1 in samples from 人. It has been validated for WB.

Immunoblot of Sf9 insect cell lysate. (PDPK1 抗体 (AA 301-312))

1 image

产品编号 ABIN116939

发货至: 中国

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Quick Overview for PDPK1 抗体 (AA 301-312) (ABIN116939)

抗原

See all PDPK1 抗体

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This PDPK1 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product PDPK1 Primary Antibody

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AA 301-312

序列

Peptide sequence: R-K-I-Q-E-V-W-R-Q-R-Y-Q-S

特异性

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is primarily directed against 60,000 MW human PDK-1 and is useful in determining its presence in various assays.

纯化方法

Immunoaffinity chromatography.

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 301-312 of PDK-1 (genome data base accession number AC005591).
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 150-429) antibody
PDPK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-429) antibody
PDPK1 适用: 人 WB, PLA 宿主: 小鼠 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-150) antibody
PDPK1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E4G11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody
PDPK1 适用: 人, 大鼠, 猴 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 4A11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 524-556), (C-Term) antibody
PDPK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 83-338) antibody
PDPK1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E2 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody
KD Validated PDPK1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB5425 unconjugated Recombinant Antibody

应用备注

This affinity purified antibody detects human PDK-1 and has been tested for use in ELISAand immunoblotting. Although untested, this reagent may be useful for Immunohistochemistry andImmunoprecipitation. It recognizes 60,000 MW human PDK-1. Reactivity in otherimmunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and Enhanced Chemiluminescence for detection. A workingdilution range of 1: 750 is suggested to detect PDK-1 in lysates derived from Sf9 insect cellsinfected with virus expressing myc-tagged human PDK1. In this experiment, approx. 10 μg of bothan untransfected sf9 cell lysate (not shown) and PDK-1 virus-infected Sf9 lysate wereseparated on a 10 % gel by SDS-PAGE followed by transfer to nitrocellulose. Primaryantibody reaction proceeded overnight at 4C using a 1: 750 dilution of the antibody in 1 %non-fat milk. This antibody predominantly recognizes a 60 kDa band corresponding tohuman PDK-1.

限制

仅限研究用
浓度

1.3 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store the antibody (in aliquots) at -20 °C.
抗原

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

别名

PDPK1

背景

PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1) phosphorylates AGC kinases. PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1

基因ID

5170

NCBI登录号

NP_002604

UniProt

O15530

途径

PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals