Recombinant TUBB 抗体
Our Local Distributor
北京 101111
Quick Overview for Recombinant TUBB 抗体 (ABIN1169288)
抗原
See all TUBB 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- anti-beta-Tubulin, mAb (rec.) (S11B)
-
特异性
- Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin.
-
产品特性
- Recombinant Antibody. Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin. Species cross-reactivity: Drosophila, Human, Monkey, Mouse, Pig, Rat. Clone: S11B. Isotype: Human IgG2lambda. Applications: ELISA, ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
-
纯化方法
- Purified from HEK 293 cell culture supernatant.
-
纯度
- >95 % (SDS-PAGE)
-
免疫原
- Full length tubulin from pig brain.
-
亚型
- IgG2 lambda
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
anti-beta-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
+4°C
-20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
-
有效期
- 12 months
-
-
- TUBB (Tubulin, beta (TUBB))
-
别名
- beta-Tubulin
-
背景
-
Alternate Names/Synonyms: Tubulin beta Chain, Tubulin beta-5 chain
Product Description: anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
-
途径
- Microtubule Dynamics, M Phase
抗原
-