NS3 抗体
This 小鼠 单克隆 antibody specifically detects NS3 in WB 和 ICC. It exhibits reactivity toward Hepatitis C Virus (HCV). It has been mentioned in 6+ publications
(6 references)
产品编号 ABIN1169266
发货至:
中国
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Our Local Distributor
中国
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北京 101111
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Quick Overview for NS3 抗体 (ABIN1169266)
抗原
NS3
适用
Hepatitis C Virus (HCV)
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunocytochemistry (ICC)
克隆位点
1B6
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特异性
- Recognizes HCV NS3 (genotype 1a and 1b) (epitope mapped to NS3 aa 160-193), recognizes NS3 alone or the NS3-4A complex.
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交叉反应
- Hepatitis C Virus (HCV)
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交叉反应 （详细）
- Does not cross-react with HCV NS3 (genotype 2a).
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纯化方法
- Purified from concentrated hybridoma tissue culture supernatant.
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纯度
- >95 % (SDS-PAGE)
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免疫原
- Recombinant hepatitis C virus (HCV) NS3 serine protease domain.
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亚型
- IgG1
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. -
有效期
- 12 months
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: "Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus." in: Nature, Vol. 437, Issue 7062, pp. 1167-72, (2005) (PubMed).
: "Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2561-6, (2005) (PubMed).
: "Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes." in: Journal of virology, Vol. 78, Issue 14, pp. 7400-9, (2004) (PubMed).
: "Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons." in: Journal of virology, Vol. 77, Issue 9, pp. 5487-92, (2003) (PubMed).
: "Protein-protein interactions between hepatitis C virus nonstructural proteins." in: Journal of virology, Vol. 77, Issue 9, pp. 5401-14, (2003) (PubMed).
: "Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines." in: Journal of virology, Vol. 74, Issue 5, pp. 2293-304, (2000) (PubMed).
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: "Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus." in: Nature, Vol. 437, Issue 7062, pp. 1167-72, (2005) (PubMed).
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- NS3
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物质类
- Viral Protein
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背景
- The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
抗原
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