Polyglutamylation 抗体 (Biotin)
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北京 101111
Quick Overview for Polyglutamylation 抗体 (Biotin) (ABIN1169177)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-Polyglutamylation Modification, mAb (GT335) (Biotin)
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特异性
- Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin.
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产品特性
- Monoclonal Antibody. Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin. Isotype: Mouse IgG1kappa. Clone: GT335. Applications: ICC, IHC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and be involved in cell cycle and cell proliferation.
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纯化方法
- Purified from concentrated hybridoma tissue culture supernatant.
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纯度
- >95 % (SDS-PAGE)
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免疫原
- Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
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亚型
- IgG1 kappa
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anti-Polyglutamylation antibody
适用: Various Species ICC, IHC, IP, WB, IEM 宿主: 小鼠 Monoclonal GT335 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Recognizes most forms of polyglutamylated tubulin (alpha- and beta-tubulin), independent of the length of the glutamate side chains. No specificity to particular tubulin isoforms nor to tubulin from particular species are observed. Detects also other (poly)glutamylated proteins. Since no consensus modification site is known for protein (poly)glutamylation, the detection is not sequence-specific. However, an acidic environment of the modification site is required. The use of the antibody at too high concentrations obscures its specificity in immunofluorescence.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- In PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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+4°C
-20°C
Stable for at least 1 year after receipt when stored at -20°C.
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有效期
- 12 months
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- Polyglutamylation
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背景
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Alternate Names/Synonyms: Polyglutamylated Tubulin, Glutamylated Tubulin, Postranslational Protein Glutamylation
Product Description: Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and be involved in cell cycle and cell proliferation.
抗原
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