Beta-Phosphoglucomutase 抗体
This anti-Beta-Phosphoglucomutase antibody is a 山羊 多克隆 antibody detecting Beta-Phosphoglucomutase in WB, EIA 和 IP. Suitable for Lactococcus lacti.
Quick Overview for Beta-Phosphoglucomutase 抗体 (ABIN116917)
抗原
Beta-Phosphoglucomutase
(Phosphoglucomutase, beta-)
适用
Lactococcus lacti
宿主
山羊
克隆类型
多克隆
标记
This Beta-Phosphoglucomutase antibody is un-conjugated
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)
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特异性
- This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified beta-Phosphoglucomutase [Lactococcus lacti]. Cross reactivity against beta-Phosphoglucomutase from other sources is unknown.
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纯化方法
- Multistep process.
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免疫原
- beta-Phosphoglucomutase [Lactococcus lacti].
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anti-Phosphoglucomutase, beta- antibody (Biotin)
适用: Lactococcus lacti WB, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Phosphoglucomutase, beta- antibody适用: Lactococcus lacti WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 mg ofbeta-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 15,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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