HCV 1b NS5B 抗体
(6 references)Quick Overview for HCV 1b NS5B 抗体 (ABIN1169141)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity).
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交叉反应
- Hepatitis C Virus (HCV)
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交叉反应 （详细）
- It does not recognize genotype 2a.
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纯化方法
- Purified from concentrated hybridoma tissue culture supernatant.
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纯度
- >95 % (SDS-PAGE)
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免疫原
- Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a).
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亚型
- IgG2b
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anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 111-130) antibody
HCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IF 宿主: CHO Cells Monoclonal 9A2 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 111-130) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 9A2 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 92-105) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP 宿主: 小鼠 Monoclonal 6B12 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 95-105) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP 宿主: 小鼠 Monoclonal 7G8 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 77-86) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) WB, ELISA, IP 宿主: 小鼠 Monoclonal 6G5 unconjugated
anti-Hepatitis C Virus NS5b (HCV NS5b) (AA 1-14) antibodyHCV NS5b 适用: Hepatitis C Virus (HCV) ELISA, IP 宿主: 小鼠 Monoclonal 8B2 unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. -
有效期
- 12 months
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: "Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2561-6, (2005) (PubMed).
: "Quantitative analysis of the hepatitis C virus replication complex." in: Journal of virology, Vol. 79, Issue 21, pp. 13594-605, (2005) (PubMed).
: "Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3." in: Journal of virology, Vol. 79, Issue 11, pp. 6868-81, (2005) (PubMed).
: "Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells." in: Journal of virology, Vol. 77, Issue 3, pp. 2029-37, (2003) (PubMed).
: "Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 235-40, (2003) (PubMed).
: "Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 593-601, (2002) (PubMed).
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: "Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2561-6, (2005) (PubMed).
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- HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))
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物质类
- Viral Protein
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背景
- The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
抗原
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