N-Acetyl-D-Mannosamine Dehydrogenase 抗体
The 山羊 多克隆 anti-N-Acetyl-D-Mannosamine Dehydrogenase antibody (ABIN116910) specifically detects N-Acetyl-D-Mannosamine Dehydrogenase in EIA, IP 和 WB.
The antibody is reactive with E. coli samples.
产品编号 ABIN116910
发货至:
中国
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Contact Info
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中国
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Quick Overview for N-Acetyl-D-Mannosamine Dehydrogenase 抗体 (ABIN116910)
抗原
N-Acetyl-D-Mannosamine Dehydrogenase
适用
E. coli
宿主
山羊
克隆类型
多克隆
应用范围
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Key Features
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- NLRP3 antibody for reliable recognition of mouse and human NLRP3/NALP3
- This un-conjugated monoclonal antibody has 60 PubMed citations
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特异性
- This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant]. Cross reactivity against N-Acyl Mannosamine-1-Dehydrogenase from other sources is unknown.
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纯化方法
- Multistep process.
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免疫原
- N-Acyl Mannosamine-1-Dehydrogenase [E.coli Recombinant].
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions. This product has been assayed against 1.0 μg of N-AcylMannosamine-1-Dehydrogenase [E. coli Recombinant] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product:
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent)
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浓度
- 90.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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