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Recombinant alpha Tubulin 抗体

This anti-alpha Tubulin antibody is a Human 单克隆 antibody detecting alpha Tubulin in WB 和 ICC. Suitable for 人, 小鼠 和 Cow. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN1169084
发货至: 中国

Quick Overview for Recombinant alpha Tubulin 抗体 (ABIN1169084)

抗原

See all alpha Tubulin (TUBA1) 抗体
alpha Tubulin (TUBA1)

抗体类型

Recombinant Antibody

适用

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人, 小鼠, Cow

宿主

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Human

克隆类型

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单克隆

标记

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This alpha Tubulin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC)

克隆位点

F2C
  • 特异性

    Recognizes mouse, bovine and human alpha-tubulin.

    交叉反应

    牛, 人, 小鼠

    纯化方法

    Purified from HEK 293 cell culture supernatant.

    纯度

    >95 % (SDS-PAGE)

    免疫原

    Bovine brain tubulin.

    亚型

    IgG2
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    In PBS containing 10 % glycerol and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

    有效期

    12 months
  • Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

    Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

  • 抗原

    alpha Tubulin (TUBA1)

    别名

    alpha-Tubulin

    背景

    Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

    途径

    Microtubule Dynamics
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