Recombinant Golgin B1 (GOLGB1) 抗体
(2 references)Quick Overview for Recombinant Golgin B1 (GOLGB1) 抗体 (ABIN1169059)
抗原
See all Golgin B1 (GOLGB1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes human and mouse giantin.
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交叉反应
- 人, 小鼠
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纯化方法
- Purified from HEK 293 cell culture supernatant.
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纯度
- >95 % (SDS-PAGE)
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免疫原
- Human recombinant giantin.
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亚型
- IgG2
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anti-Golgin B1 (GOLGB1) (AA 751-850) antibody
GOLGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Golgin B1 (GOLGB1) (AA 1-427) antibodyGOLGB1 适用: 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Golgin B1 (GOLGB1) antibodyGOLGB1 适用: 小鼠, 大鼠 WB, IHC, ICC 宿主: 豚鼠 Polyclonal unconjugated
anti-Golgin B1 (GOLGB1) (AA 1764-1869) antibodyGOLGB1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Golgin B1 (GOLGB1) (AA 3-91) antibodyGOLGB1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6D4 unconjugated
anti-Golgin B1 (GOLGB1) (AA 751-850) antibody (AbBy Fluor® 488)GOLGB1 适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 488
anti-Golgin B1 (GOLGB1) (N-Term) antibodyGOLGB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Golgin B1 (GOLGB1) antibodyGOLGB1 适用: 人 DB 宿主: 小鼠 Monoclonal 2388C3a unconjugated
anti-Golgin B1 (GOLGB1) (N-Term) antibodyGOLGB1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C. -
有效期
- 12 months
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: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).
: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).
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: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).
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- Golgin B1 (GOLGB1)
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别名
- Giantin
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背景
- Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
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UniProt
- Q14789
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途径
- SARS-CoV-2 Protein Interactome
抗原
-
