Laminin 抗体 (Biotin)
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北京 101111
Quick Overview for Laminin 抗体 (Biotin) (ABIN116904)
抗原
See all Laminin (LN) 抗体适用
宿主
克隆类型
标记
应用范围
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产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
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纯化方法
- Immunoaffinity chromatography.
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免疫原
- Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.
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anti-Laminin (LN) (AA 901-1000) antibody
LN 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Laminin (LN) antibodyLN 适用: 人, 兔, Avian, 小鸡, 犬, 鸭 IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Laminin (LN) (AA 31-270) antibodyLN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D9G5 unconjugated
anti-Laminin (LN) antibodyLN 适用: 人, 小鼠, 大鼠, 兔, 绵羊 WB, IF, ICC, IHC (p), IHC (fro), Func 宿主: 兔 Polyclonal unconjugated
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应用备注
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Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Laminin at a dilution of 1: 5,000 to 1: 10,000. This product was also assayedagainst 1.0 μg of Laminin in a standard capture ELISA using Peroxidase ConjugatedStreptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 8,000 of the stockconcentration is suggested for this product. For Immunohistochemistry on paraffinembedded tissue dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at -20 °C.
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- Laminin (LN)
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别名
- Laminin
抗原
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