Maltose Phosphorylase 抗体
This 山羊 多克隆 antibody specifically detects Maltose Phosphorylase in WB, IP 和 EIA. It exhibits reactivity toward E. coli.
产品编号 ABIN116896
发货至:
中国
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Quick Overview for Maltose Phosphorylase 抗体 (ABIN116896)
抗原
Maltose Phosphorylase
适用
E. coli
宿主
山羊
克隆类型
多克隆
标记
This Maltose Phosphorylase antibody is un-conjugated
应用范围
Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Maltose Phosphorylase [E.coli].
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anti-Maltose Phosphorylase antibody
适用: E. coli WB, IP, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of MaltosePhosphorylase [E. coli] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 20,000 to 1: 100,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Maltose Phosphorylase
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背景
- Maltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism.
抗原
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