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Pancreatic Islet Lysate 抗体

The 犬 多克隆 anti-Pancreatic Islet Lysate antibody (ABIN116793) specifically detects Pancreatic Islet Lysate in WB 和 EIA. The antibody is reactive with Pig samples.
产品编号 ABIN116793
发货至: 中国
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Quick Overview for Pancreatic Islet Lysate 抗体 (ABIN116793)

抗原

Pancreatic Islet Lysate

适用

Pig

宿主

克隆类型

多克隆

应用范围

Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 纯化方法

    Multi-step process.

    免疫原

    Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.
  • 应用备注

    Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 μg of Pancreatic IsletLysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. Aworking dilution of 1: 50 to 1: 100 is suggested for this product. This product has beenassayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane byImmunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer tonitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG[H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1: 50 to1: 200 is suggested for this product.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL (by UV absorbance at 280 nm)

    缓冲液

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Dilute only prior to immediate use

    储存条件

    -20 °C

    储存方法

    Store vial at -70 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
  • 抗原

    Pancreatic Islet Lysate
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