Pancreatic Islet Lysate 抗体
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北京 101111
Quick Overview for Pancreatic Islet Lysate 抗体 (ABIN116793)
抗原
适用
宿主
克隆类型
应用范围
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纯化方法
- Multi-step process.
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免疫原
- Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.
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应用备注
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Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 μg of Pancreatic IsletLysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. Aworking dilution of 1: 50 to 1: 100 is suggested for this product. This product has beenassayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane byImmunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer tonitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG[H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1: 50 to1: 200 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use
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储存条件
- -20 °C
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储存方法
- Store vial at -70 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
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- Pancreatic Islet Lysate
抗原
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