Luciferase 抗体
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北京 101111
Quick Overview for Luciferase 抗体 (ABIN116768)
抗原
See all Luciferase 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Luciferase [Photobacterium fischerii]
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anti-Luciferase antibody
适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 6B8 unconjugated
anti-Luciferase antibody适用: Firefly ELISA, ICC, IF (p), IF (cc), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA, IHC, FM 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, IP 宿主: 小鼠 Monoclonal 2D4 unconjugated
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/5,000-1/25,000. Western Blot: 1/1,000-1/5,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 μg of Luciferase [Photobacterium fischerii]in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 16,000 of thereconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % sodium azide as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Luciferase
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背景
- Synonyms: Alkanal monooxygenase alpha chain, LUXA
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基因ID
- 553611
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UniProt
- P09140
抗原
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