Luciferase 抗体
Quick Overview for Luciferase 抗体 (ABIN116764)
抗原
See all Luciferase 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Luciferase [Firefly].
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anti-Luciferase antibody
适用: Firefly ELISA, IF (p), IF (cc), IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA, IHC, FM 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase antibody适用: Firefly WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase (C-Term) antibody适用: Photinus pyralis WB 宿主: 山羊 Polyclonal unconjugated
anti-Luciferase (N-Term) antibody适用: Photinus pyralis WB, IHC 宿主: 小鼠 Monoclonal Luci17 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, IP 宿主: 小鼠 Monoclonal 2D4 unconjugated
anti-Luciferase antibody适用: Photinus pyralis WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Immunhistochemistry and most Immunological methods requiring high titer andspecificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Luciferase[Firefly] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature. A working dilution of 1: 20000 to1: 100000 of the reconstitution concentration is suggested for this product. For Western Blot, a recommended working dilution is 1: 2000 - 1: 10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 95.0 mg/mL (by Refractometry)
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缓冲液
- PBS, pH 7.2 containing 0.01 % sodium azides as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Luciferase
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背景
- Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.Synonyms: Luciferin 4-monooxygenase
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基因ID
- 7054
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UniProt
- P08659
抗原
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