Glucose-6-Phosphate Dehydrogenase 抗体
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Quick Overview for Glucose-6-Phosphate Dehydrogenase 抗体 (ABIN116721)
抗原
See all Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides].
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Conjugation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofGlucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat)and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 8,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 95.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use . Avoid cycles of freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at -20 °C prior to opening. This product is stable for one month at 2-8 °C as an undiluted liquid. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature.
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- Glucose-6-Phosphate Dehydrogenase (G6PD)
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别名
- G6PD
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背景
- Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase
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基因ID
- 1245
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UniProt
- P11411
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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