Glucose-6-Phosphate Dehydrogenase 抗体
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Quick Overview for Glucose-6-Phosphate Dehydrogenase 抗体 (ABIN116713)
抗原
See all Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glucose Dehydrogenase [Bacillus]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Glucose Dehydrogenase [Bacillus].
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of GlucoseDehydrogenase [Bacillus] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Goat IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,500 to 1: 32,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. For extended storage, aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
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- Glucose-6-Phosphate Dehydrogenase (G6PD)
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别名
- Glucose Dehydrogenase
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背景
- Glucose Dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.Synonyms: BSU03930, Glucose 1-dehydrogenase, gdh
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基因ID
- 938261
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NCBI登录号
- NP_388275
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UniProt
- P12310
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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