CELA1 抗体
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- 抗原 See all CELA1 抗体
- CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))
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适用
- Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CELA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity against Elastase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
- Elastase [Porcine Pancreas].
- Top Product
- Discover our top product CELA1 Primary Antibody
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anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 40-258) antibody
CELA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 159-257) antibodyCELA1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 44-259) antibodyCELA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 1-258) antibodyCELA1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (AA 80-129) antibodyCELA1 适用: 人, 小鼠, Pig, 大鼠, 兔, Cow, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) (N-Term) antibodyCELA1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chymotrypsin-Like Elastase Family, Member 1 (CELA1) antibodyCELA1 适用: 人 WB, EIA, IHC (p) 宿主: 小鼠 Monoclonal 4H5 unconjugated
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- 应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: : This product has been assayed against 1.0 μg of Elastase[Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
- 浓度
- 80.0 mg/mL (by Refractometry)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.
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- 抗原
- CELA1 (Chymotrypsin-Like Elastase Family, Member 1 (CELA1))
- 别名
- Elastase-1 (CELA1 产品)
- 别名
- ELA1 antibody, 1810009A17Rik antibody, 1810062B19Rik antibody, Ela-1 antibody, Ela1 antibody, PC-TsF antibody, ela1 antibody, ELS1 antibody, Elastase-1 antibody, ELAI1 antibody, RATELAI1 antibody, chymotrypsin like elastase family member 1 antibody, chymotrypsin-like elastase family, member 1 antibody, CELA1 antibody, Cela1 antibody, cela1 antibody
- 背景
- Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its ability to hydrolyse native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of a single polypeptide chain with a molecular weight of 29.5 kDa. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues. Elastase 1 is not a pancreatic enzyme: clinical literature that describes human elastase 1 activity in the pancreas is actually referring to elastase 2A.Synonyms: CELA1, Chymotrypsin-like elastase family member 1, ELA1
- 基因ID
- 396766
- NCBI登录号
- NP_998988
- UniProt
- P00772
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