Choline Oxidase 抗体
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北京 101111
Quick Overview for Choline Oxidase 抗体 (ABIN116681)
抗原
适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Choline Oxidase [Alcaligenes species].
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anti-Choline Oxidase antibody
适用: Alcaligenes WB, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Choline Oxidase antibody (HRP)适用: Alcaligenes WB, ELISA, IP 宿主: 山羊 Polyclonal HRP
anti-Choline Oxidase antibody (Biotin)适用: Alcaligenes WB, ELISA, IP 宿主: 山羊 Polyclonal Biotin
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Choline Oxidase[Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 32,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- Choline Oxidase
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背景
- Choline Oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.
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基因ID
- 512
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UniProt
- P16101
抗原
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