Catalase 抗体
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北京 101111
Quick Overview for Catalase 抗体 (ABIN116677)
抗原
See all Catalase (CAT) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Catalase from Bovine liver.
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Catalase [Bovineliver] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (R1364HRP) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 26,000 to 1: 142,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 5.0 mL of deionized water (or equivalent).
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浓度
- 85.0 mg/mL (by Refractometry)
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缓冲液
- 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing no preservative.
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储存液
- Without preservative
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- Catalase (CAT)
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别名
- Catalase
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背景
- Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.Synonyms: CAT, Peroxisome Marker
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基因ID
- 531682
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NCBI登录号
- NP_001030463
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UniProt
- P00432
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途径
- Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism
抗原
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