Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
特异性
This antibody detects porcine Carbxypeptidase B. Cross reactivity against Carboxypeptidase B from other tissues and species may occur but have not been specifically determined.Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carboxypeptidase B [porcine pancreas].
纯化方法
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western Blot: 1/500-1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
溶解方式
Restore with 2.0 mL of deionized water (or equivalent).
浓度
90.0 mg/mL (by Refractometry)
缓冲液
0.02 M Potassium phosphate, 0.12 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide, None
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
Carboxypeptidase catalyzes the hydrolysis of the basic amino acids lysine, arginine and ornithine from the C terminal position in polypeptides. It occurs as a zymogen in the pancreas in most vertebrates.Synonyms: CPB, CPB1, PASP, PCPB, Pancreas-specific protein