CA2 抗体
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北京 101111
Quick Overview for CA2 抗体 (ABIN116657)
抗原
See all CA2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects human Carbonic anhydrase 2. Cross reactivity against Carbonic anhydrase 2 from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [human erythrocytes].
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination
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免疫原
- Carbonic Anhydrase II from Human Erythrocytes. Genename: CA2
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anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibody
CA2 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyCA2 适用: 犬 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 1-260) antibodyCA2 适用: 小鸡 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) (C-Term) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase II (CA2) antibodyKD Validated CA2 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3300 unconjugated Recombinant Antibody
anti-Carbonic Anhydrase II (CA2) (AA 54-83), (N-Term) antibodyCA2 适用: 人, 小鼠 WB, IF 宿主: 兔 Polyclonal RB18368 unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 209-238), (C-Term) antibodyCA2 适用: 人 WB 宿主: 兔 Polyclonal RB18369 unconjugated
anti-Carbonic Anhydrase II (CA2) (AA 2-260) antibodyCA2 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS 宿主: 小鼠 Monoclonal 10E11 unconjugated
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA (1/115,000) and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of CarbonicAnhydrase II [Human Erythrocytes] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) R1364HRP and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 to 1: 5,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 80.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium phosphate, 0.12 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide, None
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE! This antibody should be protected from light as the PE-labelled product is photosensitive.
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- CA2 (Carbonic Anhydrase II (CA2))
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别名
- Carbonic Anhydrase 2
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背景
- Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II
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基因ID
- 760
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NCBI登录号
- NP_000058
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UniProt
- P00918
抗原
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