CA1 抗体
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北京 101111
Quick Overview for CA1 抗体 (ABIN116653)
抗原
See all CA1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined.
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纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Carbonic Anhydrase I [Human Erythrocytes]
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anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibody
CA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal M1 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 9-261) antibodyCA1 适用: 人, 大鼠, 小鼠 WB, IHC, FACS 宿主: 小鼠 Monoclonal 4D5 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 2-261) antibodyCA1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F1 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal M2 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 60-90), (N-Term) antibodyCA1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18358 unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 9-261) antibodyCA1 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (Internal Region) antibodyCA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (AA 1-261) antibodyCA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase I (CA1) (N-Term) antibodyCA1 适用: 人, Cow, 豚鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Carbonic Anhydrase I [HumanErythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to1: 5,000 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 100.0 mg/mL (by Refractometry)
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缓冲液
- 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4, with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
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- CA1 (Carbonic Anhydrase I (CA1))
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别名
- Carbonic Anhydrase 1
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背景
- Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.Synonyms: CA-I, CA1, CAB, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I
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基因ID
- 759
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NCBI登录号
- NP_001122301
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UniProt
- P00915
抗原
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