Bilirubin Oxidase 抗体
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北京 101111
Quick Overview for Bilirubin Oxidase 抗体 (ABIN116649)
抗原
适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Bilirubin Oxidase [Myrothecium verrucaria].
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anti-Bilirubin Oxidase antibody (HRP)
适用: Myrothecium verrucaria WB, IP, ELISA 宿主: 山羊 Polyclonal HRP
anti-Bilirubin Oxidase antibody适用: Myrothecium verrucaria WB, IP, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Bilirubin Oxidase antibody (Biotin)适用: Myrothecium verrucaria WB, IP, ELISA 宿主: 山羊 Polyclonal Biotin
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of BilirubinOxidase [Myrothecium verrucaria] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 7,500 to 1: 35,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- Bilirubin Oxidase
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背景
- Bilirubin Oxidase is an enzyme that catalyses the oxidation of bilirubin to biliverdin and other substrates. This enzyme has been expected to be used in the field of clinical pathology for the enzymatic determination of total bilirubin in serum and the treatment of jaundice and hyperbilirubinemia.
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基因ID
- 5532
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UniProt
- Q12737
抗原
-
