Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E. coli]. Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined.
纯化方法
Purified from monospecific antiserum by Delipidation and Defribination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/65,000. Western blot: 1/500-1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
溶解方式
Restore with 2.0 mL of deionized water (or equivalent).
浓度
80 mg/mL (by refractometry)
缓冲液
0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % Sodium azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.
抗原
GLB1
(Galactosidase, beta 1 (GLB1))
别名
beta Galactosidase (GAL)
背景
Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag