L-Asparaginase 1 抗体
适用: E. coli
WB, IP, ELISA
宿主: 兔
Polyclonal
unconjugated
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- 抗原
- L-Asparaginase 1
- 适用
- E. coli
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), ELISA
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Asparaginase [Escherichia coli]. Cross reactivity against Asparaginase from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Prepared from monospecific antiserum by Delipidation and Defibrination.
- 免疫原
- Asparaginase [Escherichia coli].
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- 应用备注
- Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended DilutionsELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with 2.0 mL of deionized water or equivalent.
- 浓度
- 65.0 mg/mL (by Refractometry)
- 缓冲液
- 0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 without preservatives or stabilizers.
- 储存液
- Without preservative
- 注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20°C. For extended storage aliquot contents and freeze at -20°C or below.
- 有效期
- 12 months
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- 抗原
- L-Asparaginase 1
- 背景
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Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
Alternate names: L-ASNase I, L-asparaginase I, L-asparagine amidohydrolase I, ansA - 基因ID
- 946278
- NCBI登录号
- AP_002386
- UniProt
- P0A962
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