L-Ascorbate Oxidase 抗体
The 兔 多克隆 anti-L-Ascorbate Oxidase antibody is suitable to detect L-Ascorbate Oxidase in samples from Pumpkin. It has been validated for WB, EIA 和 IP.
产品编号 ABIN116624
发货至:
中国
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中国
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北京 101111
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Quick Overview for L-Ascorbate Oxidase 抗体 (ABIN116624)
抗原
L-Ascorbate Oxidase
适用
Pumpkin
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Ascorbate Oxidase [cucurbita].
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: : This product has been assayed against 1.0 μg of AscorbateOxidase [cucurbita] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 6,000 to 1: 23,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 85.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- L-Ascorbate Oxidase
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背景
- Synonyms: AAO
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基因ID
- 3661
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UniProt
- P24792
抗原
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