ALDOA 抗体
Quick Overview for ALDOA 抗体 (ABIN116608)
抗原
See all ALDOA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
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纯化方法
- Prepared from monospecific antiserum by a delipidation and defibrination.
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免疫原
- Aldolase [Rabbit Muscle].
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anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 18-273) antibody
ALDOA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 1-364) antibodyALDOA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3D9-6F3 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 9-145) antibodyALDOA 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1C5B2 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 1-364) antibodyALDOA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3C12-6D11 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 9-145) antibodyALDOA 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2H2B1 unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 303-331) antibodyALDOA 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) (AA 66-95) antibodyALDOA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) antibodyALDOA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Monoclonal 7D8 unconjugated Recombinant Antibody
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) antibodyALDOA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Aldolase A, Fructose-Bisphosphate (ALDOA) antibodyALDOA 适用: 人, 兔 WB, ELISA, IHC, IF, IP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 m g of Aldolase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 12,000 of the reconstitution concentration is suggested for this product. Useapproximately 5 μL of antibody to immunoprecipitate 50 μL of protein lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))
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背景
- Synonyms: ALDA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, NY-LU-1
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基因ID
- 100009055
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NCBI登录号
- NP_001075707
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UniProt
- P00883
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途径
- Ribonucleoside Biosynthetic Process
抗原
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