Alkaline Phosphatase 抗体
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北京 101111
Quick Overview for Alkaline Phosphatase 抗体 (ABIN116596)
抗原
See all Alkaline Phosphatase (ALP) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This product was prepared from monospecific antiserum by a Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.
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纯化方法
- Delipidation and Defibrination
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免疫原
- Alkaline Phosphatase [E.coli].
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anti-Alkaline Phosphatase (ALP) antibody
KD Validated ALP 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3645 unconjugated Recombinant Antibody
anti-Alkaline Phosphatase (ALP) (AA 56-83) antibodyALP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 23GB1765 unconjugated Recombinant Antibody
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: 人 WB, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 21-35) antibodyALP 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) (AA 274-304) antibodyALP 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyCarrier-free ALP 适用: 人 IHC (p) 宿主: 小鼠 Monoclonal ALPL-597 unconjugated
anti-Alkaline Phosphatase (ALP) (AA 282-309) antibodyALP 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alkaline Phosphatase (ALP) antibodyALP 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Note: This product has been assayed against 1.0 μg of Alkaline Phosphatase [E. coli] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat) (Cat#R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to1/100,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/8,000-1/40,000Western Blot: 1/500-1/2,000
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 75.0 mg/mL (by Refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
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