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ADH1 抗体

This anti-ADH1 antibody is a 兔 多克隆 antibody detecting ADH1 in WB 和 EIA. Suitable for Saccharomyces cerevisiae.
产品编号 ABIN116588
发货至: 中国

Quick Overview for ADH1 抗体 (ABIN116588)

抗原

See all ADH1 抗体
ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

适用

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Saccharomyces cerevisiae

宿主

  • 16
  • 3

克隆类型

  • 16
  • 3
多克隆

标记

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  • 3
This ADH1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 特异性

    Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown.

    纯化方法

    Delipidation and defibrination.

    免疫原

    Full length Alcohol Dehydrogenase isolated from yeast.
  • 应用备注

    ELISA: 1/5,000-1/25,000). Western Blot: 1/500-1/2,000, expect a band approximately 37 kDa in size corresponding tomonomeric alcohol dehdrogenase in the appropriate cell lysate or extract.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Restore with 2.0 mL of deionized water (or equivalent).

    浓度

    90.0 mg/mL (by refractometry)

    缓冲液

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.
  • 抗原

    ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

    别名

    Alcohol Dehydrogenase 1

    背景

    Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.Synonyms: ADC1, ADH1, Alcohol dehydrogenase I, O0947, YADH-1, YOL086C

    基因ID

    854068

    NCBI登录号

    NP_014555

    UniProt

    P00330
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