ADH1 抗体
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北京 101111
Quick Overview for ADH1 抗体 (ABIN116588)
抗原
See all ADH1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown.
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纯化方法
- Delipidation and defibrination.
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免疫原
- Full length Alcohol Dehydrogenase isolated from yeast.
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anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) (AA 169-370) antibody
ADH1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) (AA 25-228) antibodyADH1 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibody (Biotin)ADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal Biotin
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibody (HRP)ADH1 适用: Saccharomyces cerevisiae WB, IHC, IP, ELISA 宿主: 兔 Polyclonal HRP
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibodyADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 1 (Class I) (ADH1) antibodyADH1 适用: Saccharomyces cerevisiae WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/5,000-1/25,000). Western Blot: 1/500-1/2,000, expect a band approximately 37 kDa in size corresponding tomonomeric alcohol dehdrogenase in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 90.0 mg/mL (by refractometry)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.
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- ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))
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别名
- Alcohol Dehydrogenase 1
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背景
- Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.Synonyms: ADC1, ADH1, Alcohol dehydrogenase I, O0947, YADH-1, YOL086C
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基因ID
- 854068
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NCBI登录号
- NP_014555
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UniProt
- P00330
抗原
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